Species identification of human echinococcosis using histopathology and genotyping in northwestern China
Identifieur interne : 000641 ( Main/Exploration ); précédent : 000640; suivant : 000642Species identification of human echinococcosis using histopathology and genotyping in northwestern China
Auteurs : Tiaoying Li [République populaire de Chine, Japon, Royaume-Uni] ; Akira Ito [Japon] ; Kazuhiro Nakaya [Japon] ; Jiamin Qiu [République populaire de Chine] ; Minoru Nakao [Japon] ; Ren Zhen [République populaire de Chine] ; Ning Xiao [République populaire de Chine] ; Xingwang Chen [République populaire de Chine] ; Patrick Giraudoux [France] ; Philip S. Craig [Royaume-Uni]Source :
- Transactions of The Royal Society of Tropical Medicine and Hygiene [ 0035-9203 ] ; 2008-06.
English descriptors
Abstract
Human cystic echinococcosis, caused by infection with the larval stage of Echinococcus granulosus, and alveolar echinococcosis, caused by the larval form of E. multilocularis, are known to be important public health problems in western China. Echinococcus shiquicus is a new species of Echinococcus recently described in wildlife hosts from the eastern Tibetan plateau and its infectivity and/or pathogenicity in humans remain unknown. In the current study, parasite tissues from various organs were collected post-operatively from 68 echinococcosis patients from Sichuan and Qinghai provinces in eastern China. The tissues were examined by histopathology and genotyped using DNA sequencing and PCR-RFLP. Histopathologically, 38 human isolates were confirmed as E. granulosus and 30 as E. multilocularis. Mitochondrial cob gene sequencing and PCR-RFLP with rrnL as the target gene confirmed 33 of 53 of the isolates to have the G1 genotype of sheep/dog strain of E. granulosus as the only source of infection, while the remaining 20 isolates were identified as E. multilocularis. No infections were found to be caused by E. shiquicus. Additionally, 5 of 20 alveolar echinococcosis patients were confirmed to have intracranial metastases from primary hepatic alveolar echinococcosis lesions. All these cases originated from four provinces or autonomous regions but most were distributed in Sichuan and Qinghai provinces, where high prevalence rates of human alveolar echinococcosis and cystic echinococcosis were previously documented.
Url:
DOI: 10.1016/j.trstmh.2008.02.019
Affiliations:
- France, Japon, Royaume-Uni, République populaire de Chine
- Angleterre, Bourgogne-Franche-Comté, Franche-Comté, Grand Manchester
- Besancon, Manchester, Salford
- Université de Franche-Comté, Université de Salford
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Echinococcus shiquicus is a new species of Echinococcus recently described in wildlife hosts from the eastern Tibetan plateau and its infectivity and/or pathogenicity in humans remain unknown. In the current study, parasite tissues from various organs were collected post-operatively from 68 echinococcosis patients from Sichuan and Qinghai provinces in eastern China. The tissues were examined by histopathology and genotyped using DNA sequencing and PCR-RFLP. Histopathologically, 38 human isolates were confirmed as E. granulosus and 30 as E. multilocularis. Mitochondrial cob gene sequencing and PCR-RFLP with rrnL as the target gene confirmed 33 of 53 of the isolates to have the G1 genotype of sheep/dog strain of E. granulosus as the only source of infection, while the remaining 20 isolates were identified as E. multilocularis. No infections were found to be caused by E. shiquicus. Additionally, 5 of 20 alveolar echinococcosis patients were confirmed to have intracranial metastases from primary hepatic alveolar echinococcosis lesions. All these cases originated from four provinces or autonomous regions but most were distributed in Sichuan and Qinghai provinces, where high prevalence rates of human alveolar echinococcosis and cystic echinococcosis were previously documented.</div></front></TEI><affiliations><list><country><li>France</li><li>Japon</li><li>Royaume-Uni</li><li>République populaire de Chine</li></country><region><li>Angleterre</li><li>Bourgogne-Franche-Comté</li><li>Franche-Comté</li><li>Grand Manchester</li></region><settlement><li>Besancon</li><li>Manchester</li><li>Salford</li></settlement><orgName><li>Université de Franche-Comté</li><li>Université de Salford</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Tiaoying" sort="Li, Tiaoying" uniqKey="Li T" first="Tiaoying" last="Li">Tiaoying Li</name></noRegion><name sortKey="Chen, Xingwang" sort="Chen, Xingwang" uniqKey="Chen X" first="Xingwang" last="Chen">Xingwang Chen</name><name sortKey="Qiu, Jiamin" sort="Qiu, Jiamin" uniqKey="Qiu J" first="Jiamin" last="Qiu">Jiamin Qiu</name><name sortKey="Xiao, Ning" sort="Xiao, Ning" uniqKey="Xiao N" first="Ning" last="Xiao">Ning Xiao</name><name sortKey="Zhen, Ren" sort="Zhen, Ren" uniqKey="Zhen R" first="Ren" last="Zhen">Ren Zhen</name></country><country name="Japon"><noRegion><name sortKey="Li, Tiaoying" sort="Li, Tiaoying" uniqKey="Li T" first="Tiaoying" last="Li">Tiaoying Li</name></noRegion><name sortKey="Ito, Akira" sort="Ito, Akira" uniqKey="Ito A" first="Akira" last="Ito">Akira Ito</name><name sortKey="Nakao, Minoru" sort="Nakao, Minoru" uniqKey="Nakao M" first="Minoru" last="Nakao">Minoru Nakao</name><name sortKey="Nakaya, Kazuhiro" sort="Nakaya, Kazuhiro" uniqKey="Nakaya K" first="Kazuhiro" last="Nakaya">Kazuhiro Nakaya</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Li, Tiaoying" sort="Li, Tiaoying" uniqKey="Li T" first="Tiaoying" last="Li">Tiaoying Li</name></region><name sortKey="Craig, Philip S" sort="Craig, Philip S" uniqKey="Craig P" first="Philip S." last="Craig">Philip S. Craig</name></country><country name="France"><region name="Bourgogne-Franche-Comté"><name sortKey="Giraudoux, Patrick" sort="Giraudoux, Patrick" uniqKey="Giraudoux P" first="Patrick" last="Giraudoux">Patrick Giraudoux</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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